2017/01/01 by Wei-Xian Wang, Weixian Wang, Yee‐Wai Cheung +7 · 7 citations
Materials Science · Biochemistry, Genetics and Molecular Biology · #Nanocluster Synthesis and Applications #Advanced biosensing and bioanalysis techniques #Gold and Silver Nanoparticles Synthesis and Applications
paper · doi:10.1039/c6an02417c
) was achieved for PfLDH in buffer solution, sensitive enough for practical use correlating with the clinical level of PfLDH in plasma from malaria-infected patients. Unique specificity was observed towards Plasmodium falciparum over Plasmodium vivax and human lactate dehydrogenase, as well as other non-specific proteins, by combining the use of AgNCs-dsDNA with a DNA aptamer against PfLDH. Moreover, the intrinsic mechanism was revealed in detail for the two-step luminescence response. The combination of DNA-scaffolded silver nanoclusters coupled to a selective single-stranded DNA aptamer allows for a highly specific and sensitive detection of PfLDH with significant promise for malaria diagnosis in future.