A bright, water-soluble far-red fluorescent probe for detecting thiols in both mitochondria and lysosomes in living cells

2025/01/01 by Arindam Mondal, Niharika Pareek, Abdul Raheman Khan +3 · 1 citation
Biochemistry, Genetics and Molecular Biology · Engineering · #Sulfur Compounds in Biology #Redox biology and oxidative stress #Nanoplatforms for cancer theranostics

paper · doi:10.1039/d5an00660k

Abstract

= 0.61 in the presence of GSH) upon reaction with thiols. The probe demonstrates exceptional sensitivity, with detection limits of 1.2 nM for GSH, 1.9 nM for Cys, and 4.7 nM for Hcy. QNB-DNBS enables real-time fluorescence imaging of thiols in human blood serum samples and cancer cells and effectively monitors thiol depletion during ferroptosis. These findings highlight the potential of QNB-DNBS as a powerful tool for selective and real-time thiol detection in biological systems.

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