Determination of Sinapic Acid Derivatives in Canola Extracts Using High‐Performance Liquid Chromatography

2009/10/29 by Rabie Khattab, Michael Eskin, Michel Aliani +1 · 26 citations
Biochemistry, Genetics and Molecular Biology · Chemical Engineering · Immunology and Microbiology · #Nitrogen and Sulfur Effects on Brassica #Chemical and Physical Properties in Aqueous Solutions #Tannin, Tannase and Anticancer Activities

paper · pdf · doi:10.1007/s11746-009-1486-0

Abstract

A high-performance liquid chromatographic (HPLC) method with diode array detection (DAD) was used to determine the total phenolics, including sinapic acid derivatives in canola. Ten Western Canadian canola seeds, six other commodity canola seeds, their corresponding press cakes and meals were analyzed. Seeds of European 00 rapeseed and Brassica Juncea (Indian mustard) were included for comparison. Phenolic compounds were separated using a gradient elution system of water-methanol-omicron-phosphoric acid solution with a flow rate of 0.8 ml/min. In addition to sinapine (SP) and sinapic acid (SA), sinapoyl glucose (SG) is reported in the methanolic extracts. The detection and quantification limits of these compounds were 0.20-0.40 and 0.50-0.80 mug/ml, respectively with recovery values over 98.0%. The content of total phenolics, SP, SA and SG in canola extracts ranged from 9.16 to 16.13, 6.39 to 12.28, 0.11 to 0.59 and 1.36 to 7.50 mg/g, respectively with significant differences among varieties.

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