Determination of Sinapic Acid Derivatives in Canola Extracts Using High‐Performance Liquid Chromatography

2009/10/29 by Rabie Khattab, Michael Eskin, Michel Aliani +1 · 169 citations
Biochemistry, Genetics and Molecular Biology · Chemical Engineering · Chemistry · Immunology and Microbiology · #Canola #Chemical and Physical Properties in Aqueous Solutions #Chemistry #Chromatography #Food science #High-performance liquid chromatography #Nitrogen and Sulfur Effects on Brassica #Tannin, Tannase and Anticancer Activities

paper · pdf · doi:10.1007/s11746-009-1486-0

published in Journal of the American Oil Chemists Society 87(2), 147-155 (Wiley)

openalex publication_date 2009/10/29 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

A high-performance liquid chromatographic (HPLC) method with diode array detection (DAD) was used to determine the total phenolics, including sinapic acid derivatives in canola. Ten Western Canadian canola seeds, six other commodity canola seeds, their corresponding press cakes and meals were analyzed. Seeds of European 00 rapeseed and Brassica Juncea (Indian mustard) were included for comparison. Phenolic compounds were separated using a gradient elution system of water-methanol-omicron-phosphoric acid solution with a flow rate of 0.8 ml/min. In addition to sinapine (SP) and sinapic acid (SA), sinapoyl glucose (SG) is reported in the methanolic extracts. The detection and quantification limits of these compounds were 0.20-0.40 and 0.50-0.80 mug/ml, respectively with recovery values over 98.0%. The content of total phenolics, SP, SA and SG in canola extracts ranged from 9.16 to 16.13, 6.39 to 12.28, 0.11 to 0.59 and 1.36 to 7.50 mg/g, respectively with significant differences among varieties.

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