2020/05/29 by Lin‐Shang Zhang, Mei‐Yun Chu, Min‐Hua Zong +3 · 3 citations
Biochemistry, Genetics and Molecular Biology · Engineering · #Enzyme Catalysis and Immobilization #Microbial Metabolic Engineering and Bioproduction #Biofuel production and bioconversion
paper · doi:10.1002/aocs.12373
Abstract 1,3‐Dioleoyl‐2‐palmitoylglycerol (OPO) is the main triacylglycerol species in human milk‐fat substitute. The production of OPO is of considerable research interest. In this study, a new strategy for producing OPO by fermentation with R. opacus is proposed. Chemically Interesterified fat (high oleic acid sunflower oil/hydrogenated palm oil 1.73:1 w/w), or a mixture of ethyl oleate/ethyl palmitate 2:1 (w/w) as a starting material. The highest biomass and oil content obtained were 3.3 g L −1 and 40.2% (dry cell weight), respectively. The yield of OPO was 0.62 g L −1 . The fatty acid composition of produced OPO was 55.7–59.7% 18:1 and 28.3–29.8% 16:0, and the sn ‐2 position was predominantly 16:0 (64.7–74.5%). 13 C‐nuclear magnetic resonance analysis showed that the sn ‐1,3 and sn ‐2 positions were predominately esterified by 18:1 and 16:0, respectively. OPO (47.1%), OPL (13.9%), PPO (13.1%), and PPoO (16:0–16:1–18:1) (10.3%) were the most abundant triacylglycerol species.