2024/03/26 by Ilusanya, Olusolape A.F., Olukole, Kehinde M., Olayiwola, Folasade O. +5
#Phenotypic characterization #dumpsite #microorganisms #resistant profile
paper · doi:10.60787/erjournal-11-1-793
Indiscriminate waste discharge and improper management of waste disposal facilities remains Nigeria's biggest environmental concerns as it poses substantial risk to both public health and ecosystem. This study explored how waste dumpsite affects the environment and public health. Soil, leachate, borehole water and air samples collected from a dumpsite situated in Kosefe Local Government in Lagos State, Nigeria and its environs were microbiologically analyzed. Microbial isolation and enumeration utilized Nutrient, Eosin Methylene Blue, Salmonella Shigella agar and MacConkey agar. Bacterial characterization was done phenotypically using morphological and biochemical techniques. Antibiotic susceptibility employed Kirby-Bauer disk diffusion Soil had the 7 7 highest bacteria count (3.0×10 CFU/g), followed by leachate (1.48x10 CFU/ml), and borehole 6 6 3 water (5.2x10 CFU/ml), while air had the least bacterial count (4.0x10 CFU/m ). Thirty two bacterial species belonging to seven genera were isolated and identified. Bacillusspp. occurred in all samples asides water, while Salmonella spp. and Escherichia coli were found in leachate and borehole water. Antibiotic resistance profile revealed that all the isolates were susceptible to gentamicin and ofloxacin while 38% were resistant to amoxicillin and 56% of the isolates were resistant to ceftriaxone. This calls for an urgent regulatory system on proper waste disposal in that area. Individuals living in close proximity to dumpsites are at high risk of infections caused by bacterial pathogens.