2016/09/03 by Alex Bardales, Qinggele Li, Bardales, Alex +9
Biochemistry, Genetics and Molecular Biology · Physics and Astronomy · #Advanced Fluorescence Microscopy Techniques #Advanced X-ray Imaging Techniques #Biological Physics (physics.bio-ph) #Digital Holography and Microscopy #FOS: Physical sciences #Medical Physics (physics.med-ph) #Optics (physics.optics)
paper · pdf · doi:10.48550/arxiv.1609.00795
openalex publication_date 2016/09/03 · openalex created_date 2016/09/16 · openalex updated_date 2026/07/28
In this work, we propose a structured illumination (SI) method based on a two-photon excitation (TPE) scanning laser beam. Advantages of TPE methods include optical sectioning, low photo-toxicity, and robustness in the face of sample induced scattering. We designed a novel multi-spot point spread function (PSF) for a fast, two-photon scanning SIM microscope. Our multi-spot PSF is generated with a phase retrieval algorithm. We show how to obtain the phase distribution and then simulate the effect of this distribution on a spatial light modulator (SLM), which produces the multi-spot PSF in the object plane of the microscope. We produce simulations that show the viability of this method. The results are simulated and a multi-spot PSF scanning SIM microscope is proposed.