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Structured illumination in Fresnel biprism-based digital holographic microscopy

2022/02/17 by S. Hossein S. Yaghoubi, Yaghoubi, S. Hossein S., Samira Ebrahimi +3
Engineering · Physics and Astronomy · #Advanced Optical Imaging Technologies #Digital Holography and Microscopy #FOS: Physical sciences #Image Processing Techniques and Applications #Instrumentation and Detectors (physics.ins-det) #Medical Physics (physics.med-ph) #Optics (physics.optics)

paper · pdf · doi:10.48550/arxiv.2202.08665

openalex publication_date 2022/02/17 · openalex created_date 2022/04/03 · openalex updated_date 2026/07/28

Abstract

The structured illumination (SI) architecture can be adopted in a digital holographic (DH) microscope to enhance the spatial resolution. In this paper, we propose and demonstrate a compact and simple SI method in a self-referencing common-path configuration of DH microscopy. The combination of SI pattern generated by a compact disk and common-path off-axis geometry formed by Fresnel biprism introduces a low-cost and highly stable system to image both amplitude and phase objects with a twofold improvement of the spatial resolution. Experimental results for the case of a standard test sample validate the predicted resolution enhancement compared to that of the conventional imager. The presented DH method allows for imaging living cells with strikingly improved clarity compared to the conventional DH microscopes.

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