2003/12/11 by Miklós Csürös, Csürös, Miklós, Bingshan Li +3
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Chromosomal and Genetic Variations #FOS: Biological sciences #G.2.1 #G.3 #Genomics (q-bio.GN) #Genomics and Phylogenetic Studies #J.3 #RNA and protein synthesis mechanisms #q-bio.GN
paper · pdf · doi:10.48550/arxiv.q-bio/0312017
An extended abstract is to be published at the 14th International Conference on Genome Informatics (GIW), December 14-17, 2003, Yokohama, Japan
arxiv created 2003/12/11 · openalex publication_date 2003/12/11 · arxiv updated 2009/12/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
This paper studies sequencing and mapping methods that rely solely on pooling and shotgun sequencing of clones. First, we scrutinize and improve the recently proposed Clone-Array Pooled Shotgun Sequencing (CAPSS) method, which delivers a BAC-linked assembly of a whole genome sequence. Secondly, we introduce a novel physical mapping method, called Clone-Array Pooled Shotgun Mapping (CAPS-MAP), which computes the physical ordering of BACs in a random library. Both CAPSS and CAPS-MAP construct subclone libraries from pooled genomic BAC clones.