2009/07/24 by Xuefeng Wang, Wang, Xuefeng, Ming Zhao +7
Biochemistry, Genetics and Molecular Biology · Engineering · Physics and Astronomy · #Advanced Biosensing Techniques and Applications #Advanced biosensing and bioanalysis techniques #Biological Physics (physics.bio-ph) #Biosensors and Analytical Detection #FOS: Physical sciences #physics.bio-ph
paper · pdf · doi:10.48550/arxiv.0907.4310
arxiv created 2009/07/24 · openalex publication_date 2009/07/24 · arxiv updated 2009/12/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
Mass-sensing biosensor arrays for protein detection require no fluorophores or enzyme labels. However, few mass biosensor protein arrays have demonstrated successful application in high background samples, such as serum. In this paper, we test the BioCD as a mass biosensor based on optical interferometry of antibodies covalently attached through Schiff-base reduction. We use the BioCD to detect prostate specific antigen (PSA, a biomarker of prostate cancer) in patient sera in a 96-well anti-PSA microarray. We have attained a 4 ng/ml detection limit in full serum and have measured PSA concentrations in three patient sera.