2013/01/14 by Tatjana Tchumatchenko, Tchumatchenko, Tatjana, Jonathan P. Newman +6
Biochemistry, Genetics and Molecular Biology · Neuroscience · #Biomolecules (q-bio.BM) #FOS: Biological sciences #Neural dynamics and brain function #Neurons and Cognition (q-bio.NC) #Neuroscience and Neural Engineering #Photoreceptor and optogenetics research #q-bio.BM #q-bio.NC
paper · pdf · doi:10.48550/arxiv.1301.3103
17 pages, 2 figures
openalex publication_date 2013/01/14 · arxiv created 2013/01/15 · arxiv updated 2013/01/16 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
To understand sensory processing in neuronal populations, it is necessary to deliver stimuli to the sensory organs of animals and record evoked population activity downstream. However, the pathways from sensory input to synaptic currents in cells that are several synapses removed from sensory organs are complex. Intrinsic noise and uncontrolled modulatory input from other brain regions can interfere with the delivery of well-defined stimuli. Here we investigate the ability of channelrhodopsins to deliver precise time-varying currents to neurons at any point along the sensory-motor pathway. To do this, we first deduce the amplitude response function of channelrhodopsin-2 (ChR2) using a three state Markov model of channel kinetics. With biophysically realistic parameters, this function supports a relatively broad signal passband and contains a resonance. We confirm the validity of our predicted amplitude response function using time-varying optical stimulation of excitatory neurons that express either wild type ChR2 or the ChR2(H134R) mutant. Together, our results indicate that ChR2-derived optogenetic tools are useful for delivering repeatable, time-varying currents to genetically-specified populations over a physiologically-relevant frequency band.