2024/11/09 by Jane F Emerson, Jane F. Emerson, Hao Wang +2 · 2 citations
Computer Science · Medicine · #Biomedical engineering #Clinical Laboratory Practices and Quality Control #Digital Imaging for Blood Diseases #Flow cytometry #Hematology #Hematology analyzer #Hematopoietic Stem Cell Transplantation #Immunology #Internal medicine #Materials science #Medical physics #Medicine #Microfluidics #Nanotechnology #Nuclear medicine #Optics #Pathology #Physics #Point of care #Point-of-care testing #Spectrum analyzer
paper · pdf · doi:10.1093/ajcp/aqae149
published in American Journal of Clinical Pathology 163(4), 531-544 (Oxford University Press)
openalex publication_date 2024/11/09 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/29
OBJECTIVES: A microfluidic flow cytometer-based point-of-care (POC) analyzer was validated against an in-laboratory hematology analyzer (Sysmex XN Automated Hematology System). Concordance on a full complete blood cell count (CBC) with 5-part differential, as performed by operators with no prior clinical laboratory experience, was evaluated. METHODS: We prospectively collected 376 venous blood specimens (376) from individuals with self-reported medical conditions and from apparently healthy individuals. Forty-six additional remnant specimens were acquired to ensure coverage of analytic measuring ranges. Parallel testing was performed, with up to 7 hours between testing on the POC and Sysmex XN analyzers. RESULTS: Regression analysis resulted in r values of 0.998 to 0.932 for all parameters of a 5-part differential CBC other than basophils (0.709). The mean percentage bias from the reference method, inclusive of the upper and lower reporting limits, was less than 2% for parameters other than lymphocytes (-6.4%), monocytes (25.9%), eosinophils (12.2%), and basophils (-15%). Overall agreement on abnormal flagging was 93.3%. CONCLUSIONS: The Cito CBC microflow cytometer (CytoChip Inc) provides a CBC with a 5-part differential with accuracy, precision, and abnormal flagging equivalent to a moderate-complexity hematology analyzer. It has the key features required of a POC device that can be operated in a waived setting: minimum space requirements, rapid results, single-action measurement (no sample processing or dilution), ease of use, and minimal blood volume.