2025/03/11 by Julia R. Torvi, Jonathan J. Wong, David G. Drubin +1
Biochemistry, Genetics and Molecular Biology · #DNA and Nucleic Acid Chemistry #Microtubule and mitosis dynamics
paper · doi:10.17912/micropub.biology.001577
openalex publication_date 2025/03/11 · openalex created_date 2025/05/20 · openalex updated_date 2026/07/28
Chromosome alignment on the mitotic spindle, also referred to as congression, is facilitated by translocation of side-bound chromosomes along the microtubule surface, which allows the establishment of end-on attachment of kinetochores to microtubule plus ends. We use a reconstitution assay in lysates prepared from metaphase-arrested budding yeast to show that kinetochore translocation along the lateral surface of microtubules is dependent on Stu2, a homolog of vertebrate XMAP215. Stu2 tracks both growing and shrinking microtubule ends but also colocalizes with moving lattice-bound kinetochores. In cells, we observed that Stu2 depletion impairs chromosome biorientation during metaphase.