1988/01/01 by Darlene Southworth · 2 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Biology #Botany #Cupressaceae #Gymnosperm #Photosynthetic Processes and Mechanisms #Plant Reproductive Biology #Plant and animal studies #Pollen #Sporopollenin
paper · doi:10.1002/j.1537-2197.1988.tb12156.x
openalex publication_date 1988/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/05/21
Exines of certain Gymnosperms spontaneously separate from the intine during the process of hydration preceding pollen germination. Exines of pollen of Calocedrus decurrens, Chamaecyparis lawsoniana, Juniperus occidentalis, Sequoia sempervirens , and Pseudotsuga menziesii were isolated from hydrated, autoclaved pollen. Free exines were purified by centrifugation on a discontinuous sucrose gradient of densities 1.14 to 1.27 g/ml. The outer intine dissolved on autoclaving. This method may be applicable to a wide range of genera. Purified exines are of potential use in chemical analyses of sporopollenin and in production of antibodies to exine.