2015/04/28 by Miloud El Karbane, Aziz ELalami, EL Karbane, Miloud +11
Chemical Engineering · Chemistry · #Analytical Chemistry and Chromatography #Analytical Chemistry and Sensors #Analytical Methods in Pharmaceuticals #HPLC #Methylparaben #Phenol #Propylparaben #Stability
paper · doi:10.48317/imist.prsm/morjchem-v3i3.3001
openalex publication_date 2015/04/28 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/01
A new, simple, specific, sensitive, rapid, accurate and precise RP-HPLC method was developed for the proportioning of the methylparaben and the propylparaben in pharmaceutical formulations as well as the phenol, which is their main degradation product. These parabens are widely used as preservatives in pharmaceutical and cosmetic products. The separation was performed on a C18-column using a mobile phase elution gradient (Acetate buffer 50mM pH 5/Acetonitrile). The detection was performed at a fixed wavelength (l = 271nm), with a flow rate of 1.3 ml/min. The detector response was linear in concentrations ranging from 10 to 60 μg/ml for the methylparaben, from 3 to 15 µg/ml for the propylparaben and from 0.125 to 2.5 µg/ml for the phenol. The intra and inter day variation was found to be less than 2%. The limits of quantification and of detection of the phenol are of 0.1 µg/ml and 0.03 µg/ml, respectively. The recovery ratio was equal to 101.8 % for the methylparaben, 101.2 % for the propylparaben and 98.4 % for the phenol. The proposed method was used to monitor the degradation of the parabens and the production of the phenol in studied solutions.