2024/10/30 by Ott, Jan, Ott, Vivian, Neubauer, Peter +2
#1:10 Turndown ratio #500 Naturwissenschaften und Mathematik::570 Biowissenschaften #600 Technik #Biologie #Biologie::570 Biowissenschaften #Chinese hamster ovary cells #Medizin #angewandte Wissenschaften::620 Ingenieurwissenschaften::620 Ingenieurwissenschaften und zugeordnete Tätigkeiten #fed-batch #perfusion #ultra-high cell density cryovial
paper · doi:10.14279/depositonce-21974
There are several ways to make antibody production processes more efficient and less expensive through process intensification. In this study, an ultra-high cell density working cell bank was used to inoculate production bioreactors directly with cells from cryovials. After lab-scale experiments, a fed-batch pilot-scale experiment was successfully conducted as a proof-of-concept. In addition, cells were cultivated in perfusion mode at 2 L scale. The intensification approach presented here allows the complete elimination of the inoculum production outside the production bioreactor and the creation of additional production capacity by saving time and space. In perfusion mode, an increase in bioreactor productivity of over 180% can be achieved compared to fed-batch mode.