2007/12/12 by Andrew Inglis, Inglis, Andrew, Luis Cruz +10
Biochemistry, Genetics and Molecular Biology · Computer Science · Physics and Astronomy · #Biological Physics (physics.bio-ph) #Cell Image Analysis Techniques #Digital Imaging for Blood Diseases #FOS: Biological sciences #FOS: Physical sciences #Image Retrieval and Classification Techniques #Neurons and Cognition (q-bio.NC) #physics.bio-ph #q-bio.NC
paper · pdf · doi:10.48550/arxiv.0712.1845
38 pages. Formatted for two-sided printing. Supplemental material and software available at http://physics.bu.edu/~ainglis/ANRA/
openalex publication_date 2007/12/12 · arxiv created 2008/04/30 · arxiv updated 2009/12/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
Individual locations of many neuronal cell bodies (>104) are needed to enable statistically significant measurements of spatial organization within the brain such as nearest-neighbor and microcolumnarity measurements. In this paper, we introduce an Automated Neuron Recognition Algorithm (ANRA) which obtains the (x,y) location of individual neurons within digitized images of Nissl-stained, 30 micron thick, frozen sections of the cerebral cortex of the Rhesus monkey. Identification of neurons within such Nissl-stained sections is inherently difficult due to the variability in neuron staining, the overlap of neurons, the presence of partial or damaged neurons at tissue surfaces, and the presence of non-neuron objects, such as glial cells, blood vessels, and random artifacts. To overcome these challenges and identify neurons, ANRA applies a combination of image segmentation and machine learning. The steps involve active contour segmentation to find outlines of potential neuron cell bodies followed by artificial neural network training using the segmentation properties (size, optical density, gyration, etc.) to distinguish between neuron and non-neuron segmentations. ANRA positively identifies 86[5]% neurons with 15[8]% error (mean[st.dev.]) on a wide range of Nissl-stained images, whereas semi-automatic methods obtain 80[7]%/17[12]%. A further advantage of ANRA is that it affords an unlimited increase in speed from semi-automatic methods, and is computationally efficient, with the ability to recognize ~100 neurons per minute using a standard personal computer. ANRA is amenable to analysis of huge photo-montages of Nissl-stained tissue, thereby opening the door to fast, efficient and quantitative analysis of vast stores of archival material that exist in laboratories and research collections around the world.