2016/12/13 by Rychtarikova, Renata, Steiner, Georg, Fischer, Michael B. +1
#FOS: Physical sciences #Medical Physics (physics.med-ph) #Optics (physics.optics)
paper · doi:10.48550/arxiv.1612.04368
The article demonstrates some less known principles of image build-up in diffractive microscopy and their usage in analysis unravelling the smallest localized information about the original object - an electromagnetic centroid. In fluorescence, the electromagnetic centroid is naturally at the position of the fluorophore. The usage of an information-entropic variable - a point divergence gain - is demonstrated for finding the most localized position of the object's representation, generally of the size of a voxel (3D pixel). These spatial pixels can be qualitatively classified and used for reconstruction of the 3D structures with precision comparable with electron microscopy.