Tutorial for the growth and development of Myxococcus xanthus as a Model System at the Intersection of Biology and Physics

2024/07/26 by Jesus Manuel Antúnez Domínguez, Laura Pérez García, Domínguez, Jesus Manuel Antúnez +19
Biochemistry, Genetics and Molecular Biology · #Bacterial Genetics and Biotechnology #Biological Physics (physics.bio-ph) #FOS: Physical sciences #Genomics and Phylogenetic Studies #Microbial Metabolic Engineering and Bioproduction

paper · pdf · doi:10.48550/arxiv.2407.18714

openalex publication_date 2024/07/26 · openalex created_date 2024/09/30 · openalex updated_date 2026/07/28

Abstract

Myxococcus xanthus is a unicellular organism whose cells possess the ability to move and communicate, leading to the emergence of complex collective properties and behaviours. This has made it an ideal model system to study the emergence of collective behaviours in interdisciplinary research efforts lying at the intersection of biology and physics, especially in the growing field of active matter research. Often, challenges arise when setting up reliable and reproducible culturing protocols. This tutorial provides a clear and comprehensive guide on the culture, growth, development, and experimental sample preparation of M. xanthus. Additionally, it includes some representative examples of experiments that can be conducted using these samples, namely motility assays, fruiting body formation, predation, and elasticotaxis.

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