Addition of <i>p</i>-Azido-<scp>l</scp>-phenylalanine to the Genetic Code of <i>Escherichia </i><i>c</i><i>oli</i>

2002/07/11 by Jason W. Chin, Stephen W. Santoro, Andrew B. Martin +3 · 28 citations
Chemistry · #Mass Spectrometry Techniques and Applications #Chemical Reactions and Mechanisms #Click Chemistry and Applications

paper · doi:10.1021/ja027007w

Abstract

We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response to the amber codon, TAG. The amino acid is incorporated in good yield with high fidelity and can be used to crosslink interacting proteins.

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