Addition of p-Azido-l-phenylalanine to the Genetic Code of Escherichia coli

2002/07/11 by Jason W. Chin, Stephen W. Santoro, Andrew B. Martin +3 · 739 citations
Chemistry · #Amino acid #Biochemistry #Chemical Reactions and Mechanisms #Chemistry #Click Chemistry and Applications #Escherichia coli #Fidelity #Gene #Genetic code #Machine learning #Mass Spectrometry Techniques and Applications #Phenylalanine #RNA #Selection (genetic algorithm) #Stereochemistry #Transfer RNA #Yield (engineering)

paper · doi:10.1021/ja027007w

published in Journal of the American Chemical Society 124(31), 9026-9027 (American Chemical Society)

openalex publication_date 2002/07/11 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/26

Abstract

We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response to the amber codon, TAG. The amino acid is incorporated in good yield with high fidelity and can be used to crosslink interacting proteins.

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