Bioorthogonal Swarming: In Situ Generation of Dendrimers

2020/12/16 by Yichuan Zhang, Muhammed Üçüncü, Alessia Gambardella +7 · 4 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · #RNA Interference and Gene Delivery #Click Chemistry and Applications #Chemical Synthesis and Analysis

paper · doi:10.1021/jacs.0c07869

Abstract

With the aid of bioorthogonal chemistry, we demonstrate the fabrication of synthetic dendrimers in situ around living cells. Using tetrazine dienophile and aminooxyl/hydrazide aldehyde chemistries, the density of functional groups on the dendrimers exponentially amplified intensities of fluorescent markers in antibody-targeted live cell imaging. This novel "swarming" approach highlights the power of bioorthogonal chemistry and provides a route to non-natural chemical structures on cells, paving the way for the generation of various artificial cellular nanostructures and scaffolds.

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• Exploring the Power of Metal-Free Click Transformations toward the Synthesis of Highly Functionalized Dendrimers for Applications in Drug Delivery. [europepmc]

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