2021/04/29 by Geoffrey Orgeur, Orgeur, Geoffrey, T. K. Baldwin +3
Agricultural and Biological Sciences · #Alfalfa #Ditylenchus dipsaci #Echantillonnage #Echantillonnage Ditylenchus dipsaci #Luzerne #Nematode management and characterization studies #Pré-screening par PCR #Sampling #Sampling Dityluz -Détection et la quantification du nématode des tiges #Seed Extract PCR #Sur semences de luzerne #Viability #Viabilité
paper · doi:10.15454/b8xb-zr54
openalex publication_date 2021/04/29 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/15
In Europe the stem nematode, Ditylenchus dipsaci was, until 14 December 2019, a quarantine pest on alfalfa seeds (then it will be a RNQP, Regulated Non-Quarantine Pests), and only nematode free-seeds could be marketed. The Dityluz project was based on a multiple and innovative partnership involving the stakeholders from the Seed sector (UFS and GNIS) and Public Research (ANSES, Laboratories of Pathology and Molecular Biology of GEVES) as well as scientific and technical experts (INRAE and FNAMS). A detection method on seed extracts by PCR (SE-PCR) was developed which involves the pooling of all the nematodes from a seed sample into a small volume and then to analyze it by PCR. The method was validated in a ring test where performance criteria were evaluated: the detection threshold was determined to be 1 D. dipsaci and sensitivity, specificity, accordance and concordance were determined at 100%. In addition, a method for assessing the viability of D. dipsaci was also developed and validated according to the evaluation of performance criteria. The results of this project were made available to seed companies and the Official Control Service through the organization of a workshop. Following numerous exchanges between GEVES, SOC, DGAL and ANSES, the method was made official in the Official Bulletin of the Ministry of Agriculture in November 2020 and applied to GEVES.