2024/03/12 by Kuger, Laura, Lux, Elisa-Marie, Franke, Jochen +3
#chromatography #fractionation #magnetic particle spectroscopy
paper · doi:10.18416/ijmpi.2024.2403019
Refining the size distribution of magnetic nanoparticles (MNPs) holds great potential for enhanced performance in magnetic particle imaging (MPI). This work demonstrates the coupling of a preparative-scale magnetic fieldcontrolled size fractionation technique with in-line magnetic particle spectroscopy (MPS) for the real-time assessment of magnetic nanoparticle downstream processing performance. In-line magnetic particle spectroscopy monitoring allowed for the concurrent evaluation of the amplitude spectrum and derived parameters including the harmonic ratio A5/A3, enabling real-time selection of fractions with desired characteristics during the fractionation process. Furthermore, magnetic particle spectroscopy offered increased sensitivity and more comprehensive characterization capabilities than conventional in-line analysis techniques such as light absorption spectroscopy. A theoretical model delineating the dependency of magnetic particle spectroscopy signal on core/shell size ratio was developed. These results potentially have significant implications for prospective applications in the realm of in-line magnetic particle spectroscopy analysis for real-time process control and quality assurance.