2018/10/02 by Jason Deglint, Lyndon Tang, Deglint, Jason L. +7
Biochemistry, Genetics and Molecular Biology · Environmental Science · #Biological Physics (physics.bio-ph) #Cell Image Analysis Techniques #FOS: Biological sciences #FOS: Physical sciences #Instrumentation and Detectors (physics.ins-det) #Quantitative Methods (q-bio.QM) #Water Quality Monitoring and Analysis
paper · pdf · doi:10.48550/arxiv.1810.09838
openalex publication_date 2018/10/02 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
This paper presents SAMSON, a Spectral Absorption-fluorescence Microscopy\nSystem for ON-site-imaging of algae within a water sample. Designed to be\nportable and low-cost for on-site use, the optical sub-system of SAMSON\nconsists of a mixture of low-cost optics and electronics, designed specifically\nto capture both fluorescent and absorption responses from a water sample. The\ngraphical user interface (GUI) sub-system of SAMSON was designed to enable\nflexible visualisation of algae in the water sample in real-time, with the\nability to perform fine-grained exposure control and illumination wavelength\nselection. We demonstrate SAMSON's capabilities by equipping the system with\ntwo fluorescent illumination sources and seven absorption illumination sources\nto enable the capture of multispectral data from six different algae species\n(three from the Cyanophyta phylum (blue-green algae) and three from the\nChlorophyta phylum (green algae)). The key benefit of SAMSON is the ability to\nperform rapid acquisition of fluorescence and absorption data at different\nwavelengths and magnification levels, thus opening the door for machine\nlearning methods to automatically identify and enumerate different algae in\nwater samples using this rich wealth of data.\n