2016/07/28 by Partha Pratim Mondal, Mondal, Partha Pratim
Biochemistry, Genetics and Molecular Biology · Engineering · #Advanced Electron Microscopy Techniques and Applications #Advanced Fluorescence Microscopy Techniques #Biological Physics (physics.bio-ph) #Cell Image Analysis Techniques #FOS: Physical sciences #Image Processing Techniques and Applications #Optics (physics.optics)
paper · pdf · doi:10.48550/arxiv.1608.00542
openalex publication_date 2016/07/28 · openalex created_date 2022/08/27 · openalex updated_date 2026/07/28
Fast 3D super-resolution imaging is essential for decoding rapidly occurring\nbiological processes. Encoding single molecules to their respective planes\nenable simultaneous multi-plane super-resolution volume imaging. This saves the\ndata-acquisition time and as a consequence reduce radiation-dose that lead to\nphotobleaching and other undesirable photochemical reactions. Detection and\nsubsequent identification of the locus of individual molecule (both on the\nfocal plane and off-focal planes) holds the key. Experimentally, this is\nachieved by accurate calibration of system PSF size and its natural spread in\noff-focal planes using sub-diffraction fluorescent beads. Subsequently the\nidentification and sorting of single molecules that belong to different axial\nplanes is carried out (by setting multiple cut-offs to respective PSFs).\nSimultaneous Multiplane Imaging based Localization Encoded (SMILE) microscopy\ntechnique eliminates the need for multiple z-plane scanning and thereby\nprovides a truly simultaneous multiplane super-resolution imaging.\n