BADER: Bayesian analysis of differential expression in RNA sequencing data

2014/10/17 by Matthias Katzfuß, Katzfuss, Matthias, Andreas Neudecker +5
Biochemistry, Genetics and Molecular Biology · #Applications (stat.AP) #FOS: Computer and information sciences #Methodology (stat.ME) #Molecular Biology Techniques and Applications #RNA Research and Splicing #RNA modifications and cancer

paper · pdf · doi:10.48550/arxiv.1410.4827

openalex publication_date 2014/10/17 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

Identifying differentially expressed genes from RNA sequencing data remains a challenging task because of the considerable uncertainties in parameter estimation and the small sample sizes in typical applications. Here we introduce Bayesian Analysis of Differential Expression in RNA-sequencing data (BADER). Due to our choice of data and prior distributions, full posterior inference for BADER can be carried out efficiently. The method appropriately takes uncertainty in gene variance into account, leading to higher power than existing methods in detecting differentially expressed genes. Moreover, we show that the posterior samples can be naturally integrated into downstream gene set enrichment analyses, with excellent performance in detecting enriched sets. An open-source R package (BADER) that provides a user-friendly interface to a C++ back-end is available on Bioconductor.

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