2015/05/05 by Manoj M. Varma, Varma, Manoj M.
Biochemistry, Genetics and Molecular Biology · Engineering · #Advanced Biosensing Techniques and Applications #Advanced biosensing and bioanalysis techniques #Biological Physics (physics.bio-ph) #Biosensors and Analytical Detection #FOS: Biological sciences #FOS: Physical sciences #Instrumentation and Detectors (physics.ins-det) #Quantitative Methods (q-bio.QM)
paper · pdf · doi:10.48550/arxiv.1505.01032
openalex publication_date 2015/05/05 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
Immunoassay formats applicable for clinical or point-of-care diagnostics fall into two broad classes. One which uses labeled secondary antibodies for signal transduction and the other which does not require the use of any labels. Comparison of the limits of detection (LoD) reported by these two sensing approaches over a wide range of detection techniques and target molecules in serum revealed that labeled techniques achieve 2-3 orders of magnitude better LoDs. Further, a vast majority of commercial tests and recent examples of technology translations are based on labeled assay formats. In light of this data, it is argued that extension of traditional labeled approaches and enhancing their functionality may have better clinical impact than the development of newer label-free techniques.