Detection of Ebola Virus RNA through Aerosol Sampling of Animal Biosafety Level 4 Rooms Housing Challenged Nonhuman Primates

2016/12/08 by David E. Harbourt, Sara C. Johnston, James Pettitt +2 · 1 voice · 3 citations
Medicine · #Viral Infections and Outbreaks Research #Viral Infections and Vectors #Viral gastroenteritis research and epidemiology

paper · pdf · doi:10.1093/infdis/jiw610

Abstract

Ebola virus disease is a serious illness of humans and nonhuman primates (NHPs). Direct contact has been shown to be the primary source of Ebola (EBOV) transmission. We used a high-volume air sampler to determine whether EBOV could be detected during 3 independent studies with EBOV-challenged NHPs. Viral RNA was recovered during days 9 and 10 of Study I and days 7 and 8 of Study III. Viral RNA levels were below limits of detection during all other collections. The results demonstrate that the biosafety level 4 (BSL-4) suit protects workers from aerosols in a BSL-4 environment using proper engineering and administrative controls.

Cited by

• Bioaerosols and Transmission, a Diverse and Growing Community of Practice. [europepmc]
• A Small-Particle Aerosol Model of Ebolavirus Zaire Infection in Ferrets. [europepmc]
• Platelet-like particles released from Ebola virus-infected megakaryocytic cells behave like virus-like particles. [europepmc]

Discussions

• Dear 🤡🤡🤡, Ebola virus detected in air samples from non human primates. Sincerely A very fed up observer. doi.org/10.1093/infd... [bsky, 208 points, 13 comments]

Related

• The Ebola virus – going beyond the bleeding edge
• Coordinated implementation of a conventional PCR assay to detect all Ebola and Marburg virus species in a European laboratory network
• Ebola Virus Disease (EVD)
• Assessing Concordance between RNA-Seq and NanoString Technologies in Ebola-Infected Nonhuman Primates Using Machine Learning
• Social and Contextual Issues Involving Outbreak Control of Ebola Haemorrhagic Fever