Prenatal testosterone exposure is related to sexually dimorphic facial morphology in adulthood

2015/09/23 by Andrew J. O. Whitehouse, Andrew Whitehouse, Syed Zulqarnain Gilani +10 · 191 citations
Biochemistry, Genetics and Molecular Biology · Medicine · Psychology · Social Sciences · #Anatomy #Biology #Confounding #Demographic Trends and Gender Preferences #Endocrinology #Evolutionary Psychology and Human Behavior #Femininity #Internal medicine #Masculinity #Medicine #Physiology #Psychology #Sexual Differentiation and Disorders #Sexual dimorphism #Testosterone (patch) #Umbilical cord

paper · pdf · doi:10.1098/rspb.2015.1351

published in Proceedings of the Royal Society B Biological Sciences 282(1816), 20151351 (Royal Society)

openalex publication_date 2015/09/23 · openalex created_date 2016/06/24 · openalex updated_date 2026/08/04

Abstract

Prenatal testosterone may have a powerful masculinizing effect on postnatal physical characteristics. However, no study has directly tested this hypothesis. Here, we report a 20-year follow-up study that measured testosterone concentrations from the umbilical cord blood of 97 male and 86 female newborns, and procured three-dimensional facial images on these participants in adulthood (range: 21-24 years). Twenty-three Euclidean and geodesic distances were measured from the facial images and an algorithm identified a set of six distances that most effectively distinguished adult males from females. From these distances, a 'gender score' was calculated for each face, indicating the degree of masculinity or femininity. Higher cord testosterone levels were associated with masculinized facial features when males and females were analysed together (n = 183; r = -0.59), as well as when males (n = 86; r = -0.55) and females (n = 97; r = -0.48) were examined separately (p-values < 0.001). The relationships remained significant and substantial after adjusting for potentially confounding variables. Adult circulating testosterone concentrations were available for males but showed no statistically significant relationship with gendered facial morphology (n = 85, r = 0.01, p = 0.93). This study provides the first direct evidence of a link between prenatal testosterone exposure and human facial structure.

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