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Embedding-based Instance Segmentation in Microscopy

2021/01/25 by Manan Lalit, Lalit, Manan, Pavel Tomančák +3 · 1 citation
Biochemistry, Genetics and Molecular Biology · Engineering · #Advanced Electron Microscopy Techniques and Applications #Cell Image Analysis Techniques #Computer Vision and Pattern Recognition (cs.CV) #FOS: Computer and information sciences #FOS: Electrical engineering #Image Processing Techniques and Applications #Image and Video Processing (eess.IV) #Machine Learning (cs.LG) #electronic engineering #information engineering

paper · pdf · doi:10.48550/arxiv.2101.10033

openalex publication_date 2021/01/25 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

Automatic detection and segmentation of objects in 2D and 3D microscopy data is important for countless biomedical applications. In the natural image domain, spatial embedding-based instance segmentation methods are known to yield high-quality results, but their utility for segmenting microscopy data is currently little researched. Here we introduce EmbedSeg, an embedding-based instance segmentation method which outperforms existing state-of-the-art baselines on 2D as well as 3D microscopy datasets. Additionally, we show that EmbedSeg has a GPU memory footprint small enough to train even on laptop GPUs, making it accessible to virtually everyone. Finally, we introduce four new 3D microscopy datasets, which we make publicly available alongside ground truth training labels. Our open-source implementation is available at https://github.com/juglab/EmbedSeg.

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