The new frontier of genome engineering with CRISPR-Cas9

2014/11/28 by Jennifer A. Doudna, Emmanuelle Charpentier · 7,038 citations
Biochemistry, Genetics and Molecular Biology · #Animal Genetics and Reproduction #Biology #Bottleneck #CRISPR #CRISPR and Genetic Engineering #Cas9 #Computational biology #Computer science #Gene #Genetics #Genome #Genome editing #Genome engineering #Genomics #Palindrome #RNA and protein synthesis mechanisms #Synthetic biology

paper · doi:10.1126/science.1258096

published in Science 346(6213), 1258096 (American Association for the Advancement of Science)

openalex publication_date 2014/11/28 · openalex created_date 2016/06/24 · openalex updated_date 2026/08/05

Abstract

The advent of facile genome engineering using the bacterial RNA-guided CRISPR-Cas9 system in animals and plants is transforming biology. We review the history of CRISPR (clustered regularly interspaced palindromic repeat) biology from its initial discovery through the elucidation of the CRISPR-Cas9 enzyme mechanism, which has set the stage for remarkable developments using this technology to modify, regulate, or mark genomic loci in a wide variety of cells and organisms from all three domains of life. These results highlight a new era in which genomic manipulation is no longer a bottleneck to experiments, paving the way toward fundamental discoveries in biology, with applications in all branches of biotechnology, as well as strategies for human therapeutics.

Citations

• A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

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