2015/12/29 by Peeyush Sahay, Aditya Ganju, Sahay, Peeyush +15
Biochemistry, Genetics and Molecular Biology · Engineering · #Advanced Fluorescence Microscopy Techniques #Biological Physics (physics.bio-ph) #Cell Behavior (q-bio.CB) #Cell Image Analysis Techniques #FOS: Biological sciences #FOS: Physical sciences #Optics (physics.optics) #Photoacoustic and Ultrasonic Imaging
paper · pdf · doi:10.48550/arxiv.1512.08583
openalex publication_date 2015/12/29 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/28
Intracellular structural alterations are hallmark of several disease conditions and treatment modalities. However, robust methods to quantify these changes are scarce. In view of this, we introduce a new method to quantify structural alterations in biological cells through the widely used confocal microscopy. This novel method employs optical eigenfunctions localization properties of cells and quantifies the degree of structural alterations, in terms of nano- to micron scale intracellular mass density fluctuations, in one single parameter. Such approach allows a powerful way to compare changing structures in heterogeneous cellular media irrespective of the origin of the cause. As a case study, we demonstrate its applicability in cancer detection with breast and prostate cancer cases of different tumorigenicity levels. Adding new dimensions to the confocal based studies, this technique has potentially significant applications in areas ranging from disease diagnostics to therapeutic studies, such as patient prognosis and assessing survival potential.