Natural site-directed mutagenesis might exist in eukaryotic cells

2016/03/21 by Gao-De Li, Li, Gao-De
Biochemistry, Genetics and Molecular Biology · #CRISPR and Genetic Engineering #DNA Repair Mechanisms #DNA and Nucleic Acid Chemistry #FOS: Biological sciences #Subcellular Processes (q-bio.SC)

paper · pdf · doi:10.48550/arxiv.1603.06538

openalex publication_date 2016/03/21 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

Site-directed mutagenesis refers to a man-made molecular biology method that is used to make genetic alterations in the DNA sequence of a gene of interest. But based on our recently published experimental findings, we propose that natural site-directed mutagenesis might exist in the eukaryotic cells, which is triggered by harmful agents and co-directed by special transcription hotspots and mutation-contained intranuclear primers.

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