Petersilie et al. 2024b - Figure 2

2024/12/03 by Petersilie, Laura, Kafitz, Karl W., Neu, Louis A. +4
#NATURAL SCIENCES::Biology::Cell and molecular biology::Neurobiology

paper · doi:10.25838/d5p-72

Abstract

Three-dimensional brain organoids from human pluripotent stem cells are a powerful tool for studying human neural networks. This article presents a refined protocol for generating robust brain organoid slices derived from regionalized cortical organoids and grown at the air-liquid interphase. The procedures for slicing organoids and maintaining them in long-term culture are described. We then detail approaches for quality control including evaluation of cell death and cellular identity. Finally, we describe procedures for expression of a genetically-encoded nanosensor for ATP.

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