2010/07/03 by Wiebe H. Meijer, Loknath Gidijala, Susan Fekken +7 · 3 citations
Biochemistry, Genetics and Molecular Biology · Medicine · #Fungal and yeast genetics research #Microbial Natural Products and Biosynthesis #Plant biochemistry and biosynthesis
paper · doi:10.1128/aem.02327-09
openalex publication_date 2010/07/03 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/28
In the fungus Penicillium chrysogenum, penicillin (PEN) production is compartmentalized in the cytosol and in peroxisomes. Here we show that intact peroxisomes that contain the two final enzymes of PEN biosynthesis, acyl coenzyme A (CoA):6-amino penicillanic acid acyltransferase (AT) as well as the side-chain precursor activation enzyme phenylacetyl CoA ligase (PCL), are crucial for efficient PEN synthesis. Moreover, increasing PEN titers are associated with increasing peroxisome numbers. However, not all conditions that result in enhanced peroxisome numbers simultaneously stimulate PEN production. We find that conditions that lead to peroxisome proliferation but simultaneously interfere with the normal physiology of the cell may be detrimental to antibiotic production. We furthermore show that peroxisomes develop in germinating conidiospores from reticule-like structures. During subsequent hyphal growth, peroxisome proliferation occurs at the tip of the growing hyphae, after which the organelles are distributed over newly formed subapical cells. We observed that the organelle proliferation machinery requires the dynamin-like protein Dnm1.