1983/01/01 by H. P. Ginn, L. A. Pearson, Leanne A. Pearson +4 · 1 citation
Agricultural and Biological Sciences · Computer Science · Decision Sciences · Earth and Planetary Sciences · Engineering · Environmental Science · #Aquatic Ecosystems and Phytoplankton Dynamics #Bayesian Modeling and Causal Inference #Biocrusts and Microbial Ecology #Fault Detection and Control Systems #Marine and coastal ecosystems #Risk and Safety Analysis
paper · doi:10.1128/aem.01862-09
openalex publication_date 1983/01/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/06/11
NtcA is a transcription factor that has been found in a diverse range of cyanobacteria. This nitrogen-controlled factor was focused on as a key component in the yet-to-be-deciphered regulatory network controlling microcystin production. Adaptor-mediated PCR was utilized to isolate the ntcA gene from Microcystis aeruginosa PCC 7806. This gene was cloned, and the recombinant (His-tagged) protein was overexpressed and purified for use in mobility shift assays to analyze NtcA binding to putative sites identified in the microcystin mcyA/D promoter region. Autoregulation of NtcA in M. aeruginosa was shown via NtcA binding in the upstream ntcA promoter region. The observation of binding of NtcA to the mcyA/D promoter region has direct relevance for the regulation of microcystin biosynthesis, as transcription of the mcyABCDEFGHIJ gene cluster appears to be under direct control of nitrogen.