Evaluation of Decontamination Methods and Growth Media for Primary Isolation of Mycobacterium ulcerans from Surgical Specimens

2004/12/01 by Dorothy Yeboah-Manu, Dorothy Yeboah‐Manu, Thomas Bodmer +7 · 2 citations
Medicine · #Biology #Buruli ulcer #Diagnosis and treatment of tuberculosis #Isolation (microbiology) #Medicine #Microbiology #Mycobacterium #Mycobacterium avium complex #Mycobacterium research and diagnosis #Mycobacterium ulcerans #Pathology #Patient isolation #Surgery #Tuberculosis #Tuberculosis Research and Epidemiology #University hospital #Virology

paper · doi:10.1128/jcm.42.12.5875-5876.2004

openalex publication_date 2004/12/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/28

Abstract

We evaluated four decontamination methods and one nondecontamination procedure in combination with four egg-based media for the primary isolation of Mycobacterium ulcerans from tissue specimens. With mycobacterial recovery and contamination rates of 75.6 and 2.4%, respectively, the combination of the oxalic acid decontamination method with Lowenstein-Jensen medium supplemented with glycerol yielded the best results.

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