Development of a Multiplex Real-Time PCR for Detection and Differentiation of Malignant Catarrhal Fever Viruses in Clinical Samples

2009/06/04 by Cristina W. Cunha, Lisa Otto, Naomi S. Taus +2
Medicine · Agricultural and Biological Sciences · #Herpesvirus Infections and Treatments #Animal Disease Management and Epidemiology #Animal Virus Infections Studies

paper · doi:10.1128/jcm.00997-09

Abstract

A multiplex real-time PCR was developed using a single pair of primers and fluorescent probes specific for five malignant catarrhal fever viruses and an internal positive control. The assay was able to simultaneously detect and differentiate the viruses in clinical samples with high sensitivity (97.2%) and specificity (100%).

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