2023/04/12 by Mariia Filianina, Filianina, Mariia, Maddalena Bin +17 · 1 citation
Agricultural and Biological Sciences · Earth and Planetary Sciences · Materials Science · #Biological Physics (physics.bio-ph) #Chemical Physics (physics.chem-ph) #Enzyme Structure and Function #FOS: Physical sciences #Proteins in Food Systems #Soft Condensed Matter (cond-mat.soft) #nanoparticles nucleation surface interactions
paper · pdf · doi:10.48550/arxiv.2304.05802
openalex publication_date 2023/04/12 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/04
Studying protein interactions at low temperatures has important implications for optimizing cryostorage processes of biological tissue, food, and protein-based drugs. One of the major challenges is related to the formation of ice nanocrystals which can occur even in the presence of cryoprotectants and can lead to protein denaturation. Here, using a combination of small- and wide-angle X-ray scattering (SAXS and WAXS), we investigate the structural evolution of concentrated Lysozyme solutions in a cryoprotected glycerol-water mixture upon cooling from room temperature (T=300 K) down to cryogenic temperatures (T=195 K). Upon cooling, we observe a transition near the melting temperature of the solution (T~245 K), which manifests both in the temperature dependence of the scattering intensity peak position reflecting protein-protein length scales (SAXS) and the interatomic distances within the solvent (WAXS). Upon thermal cycling, a hysteresis is observed in the scattering intensity, which is attributed to the formation of nanocrystallites in the order of 10 nm. The experimental data is well described by the two-Yukawa model, which indicates temperature-dependent changes in the short-range attraction of the protein-protein interaction potential. Our results demonstrate that the nanocrystal growth yields effectively stronger protein-protein attraction and influences the protein pair distribution function beyond the first coordination shell.