Gene–Environment Interactions in Developmental Neurotoxicity: a Case Study of Synergy between Chlorpyrifos and CHD8 Knockout in Human BrainSpheres

2021/07/01 by Sergio Modafferi, Xiali Zhong, André Kleensang +12 · 2 citations
Neuroscience · Biochemistry, Genetics and Molecular Biology · #Autism Spectrum Disorder Research #CRISPR and Genetic Engineering #Insect Resistance and Genetics

paper · doi:10.1289/ehp8580

Abstract

BACKGROUND: ) interactions. OBJECTIVES: ) and environmental exposure to an organophosphate pesticide (chlorpyrifos; CPF) in an iPSC-derived human three-dimensional (3D) brain model. METHODS: and exposed to CPF or its oxon-metabolite (CPO). Neural differentiation, viability, oxidative stress, and neurite outgrowth were assessed, and levels of main neurotransmitters and selected metabolites were validated against human data on ASD metabolic derangements. RESULTS: in BrainSpheres led to an imbalance of excitatory/inhibitory neurotransmitters and lower levels of dopamine. DISCUSSION: ) interaction in ASD. https://doi.org/10.1289/EHP8580.

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