The reconstruction of a three-dimensional structure from projections and its application to electron microscopy

1970/06/23 by Richard Anthony Crowther, R.A. Crowther, D. J. DeRosier +2 · 8 citations
Biochemistry, Genetics and Molecular Biology · Engineering · Materials Science · #Advanced Electron Microscopy Techniques and Applications #Advancements in Photolithography Techniques #Electron and X-Ray Spectroscopy Techniques

paper · doi:10.1098/rspa.1970.0119

openalex publication_date 1970/06/23 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/30

Abstract

Abstract A transmission electron micrograph is essentially a projection of the specimen in the direction of view. In order to reconstruct a three-dimensional image of the specimen, it is necessary to be able to combine data from a number of different views. A formal solution of this problem is given in terms of Fourier transforms. Its realization requires data reduction and interpolation. The final solution is given by a least squares approach, which also indicates how many views must be included to give a valid reconstruction of a given particle to a given degree of resolu­tion. Interpolation procedures of varying power are given, to be employed according to the economy with which the available data must be used. An alternative procedure is described for direct reconstruction without the use of Fourier transforms, but it is shown to be in general less practicable than the Fourier approach.

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