2022/11/22 by Gianluca Ferraro, Ferraro, Gianluca, Ylenia Gigante +11
Biochemistry, Genetics and Molecular Biology · Neuroscience · #Advanced Fluorescence Microscopy Techniques #FOS: Physical sciences #Medical Physics (physics.med-ph) #Neuroscience and Neural Engineering #Optics (physics.optics) #Retinal Development and Disorders
paper · pdf · doi:10.48550/arxiv.2211.12375
openalex publication_date 2022/11/22 · openalex created_date 2022/11/29 · openalex updated_date 2026/07/28
Phantom eye devices are designed to mimic the optical properties of the human eye; however, the retinal structures are typically emulated with synthetic tissues. Thus, it is currently impossible to exploit phantom eye devices for fluorescence microscopy experiments on cultures, or ex-vivo tissues, although these ophthalmic measurements would be of importance for many applications such as the testing of retinal biomarkers. Here we report and describe the development of a phantom eye designed to host biological samples, such as retinal cultures differentiated from human induced pluripotent stem cells. We characterized the imaging performance of the humanized phantom eye on standard biomarkers such as Alexa Fluor 532 and Alexa fluor 594.