2025/10/09 by Kang Zhu, Chatrin Chatrin, Rebecca Smith +2
Medicine · Biochemistry, Genetics and Molecular Biology · #PARP inhibition in cancer therapy #Autophagy in Disease and Therapy #Ubiquitin and proteasome pathways
paper · pdf · doi:10.1042/ebc20253040
Ubiquitination is a fundamental post-translational modification essential for nearly all cellular activities. Traditionally, ubiquitination has been understood as a protein modification, where ubiquitin (Ub) molecules are covalently attached to the lysine residues of substrate proteins, thereby modulating their function, localization, or degradation. However, recent discoveries have expanded the scope of ubiquitination beyond protein substrates. One of the examples is ubiquitination of ADP-ribose moieties on proteins or nucleic acids that leads to the formation of a dual-hybrid modification ADP-ribose-Ub (ADPr-Ub). This novel form of ubiquitination is catalyzed by Deltex ubiquitin ligases that act in concert with PARPs (Poly (ADP-ribose) polymerases), enzymes modifying their substrates by ADPr modification. This review summarizes our current knowledge of mechanisms and potential functional implications of ADPr-Ub. We also cover other examples of the interplay between ADP-ribosylation (ADPr) and ubiquitination beyond Deltex enzymes and ADPr-Ub.