1972/06/01 by E. C. Cocking, Edward C. Cocking
Biochemistry, Genetics and Molecular Biology · Agricultural and Biological Sciences · #Plant tissue culture and regeneration #Plant Reproductive Biology #Plant Molecular Biology Research
paper · doi:10.1146/annurev.pp.23.060172.000333
INTRODUCTION. • . • . • • • • . . . . . . • . . . . . • . . . . . . . . . . . • . • . . • • . • . • • • . . . . . . . . . • . . 29 IsOLATION OF PLANT CELL PROTOPLASTS..... . . . .. .. . 30 Mechanical methods for the isolation of protoplasts. . . . . . . . . . . . . . . . . . . . . . . . . 30 Enzymatic methods for the isolation of protoplasts. . . . . . . . . . . . . . . . . . . . . . . .. . 31 GROWTH AND DEVELOPMENT OF IsOLATED PROTOPLASTS . . . . . . . . . . . . . . . . • . . . ' 38 Cell wall regeneration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 Division. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 Development and possible genetic modification of isolated protoplasts and plants. . . 44