2018/08/25 by Julia Bally, Hyungtaek Jung, Cara L. Mortimer +8 · 13 citations
Biochemistry, Genetics and Molecular Biology · Agricultural and Biological Sciences · #Plant tissue culture and regeneration #Transgenic Plants and Applications #Plant Virus Research Studies
paper · doi:10.1146/annurev-phyto-080417-050141
A decade ago, the value of Nicotiana benthamiana as a tool for plant molecular biologists was beginning to be appreciated. Scientists were using it to study plant-microbe and protein-protein interactions, and it was the species of choice with which to activate plasmid-encoded viruses, screen for gene functions with virus-induced gene silencing (VIGS), and transiently express genes by leaf agroinfiltration. However, little information about the species' origin, diversity, genetics, and genomics was available, and biologists were asking the question of whether N. benthamiana is a second fiddle or virtuoso. In this review, we look at the increased knowledge about the species and its applications over the past decade. Although N. benthamiana may still be the sidekick to Arabidopsis, it shines ever more brightly with realized and yet-to-be-exploited potential.