2023/08/10 by Mark A.A. Minow, Alexandre P. Marand, Robert J. Schmitz · 24 citations
Biochemistry, Genetics and Molecular Biology · #Biology #Cell #Computational biology #Evolution and Genetic Dynamics #Forward genetics #Functional genomics #Gene #Genetic Mapping and Diversity in Plants and Animals #Genetic architecture #Genetic screen #Genetics #Genome #Genomics #Phenotype #Population #Single-cell analysis #Single-cell and spatial transcriptomics
paper · doi:10.1146/annurev-genet-022123-110824
published in Annual Review of Genetics 57(1), 297-319 (Annual Reviews)
openalex publication_date 2023/08/10 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/06
The ease and throughput of single-cell genomics have steadily improved, and its current trajectory suggests that surveying single-cell populations will become routine. We discuss the merger of quantitative genetics with single-cell genomics and emphasize how this synergizes with advantages intrinsic to plants. Single-cell population genomics provides increased detection resolution when mapping variants that control molecular traits, including gene expression or chromatin accessibility. Additionally, single-cell population genomics reveals the cell types in which variants act and, when combined with organism-level phenotype measurements, unveils which cellular contexts impact higher-order traits. Emerging technologies, notably multiomics, can facilitate the measurement of both genetic changes and genomic traits in single cells, enabling single-cell genetic experiments. The implementation of single-cell genetics will advance the investigation of the genetic architecture of complex molecular traits and provide new experimental paradigms to study eukaryotic genetics.