2014/01/01 by Günter Kampf, Kampf, Günter, Stina Degenhardt +5 · 1 citation
Biochemistry, Genetics and Molecular Biology · Medicine · #Infections and bacterial resistance #Infectious Aortic and Vascular Conditions #Hemostasis and retained surgical items
paper · doi:10.3205/dgkh000229
BACKGROUND: It has recently been reported that reusable dispensers for surface disinfection tissues may be contaminated, especially with adapted Achromobacter species 3, when products based on surface-active ingredients are used. Fresh solution may quickly become recontaminated if dispensers are not processed adequately. METHODS: We evaluated the abilities of six manual and three automatic processes for processing contaminated dispensers to prevent recolonisation of a freshly-prepared disinfectant solution (Mikrobac forte 0.5%). Dispensers were left at room temperature for 28 days. Samples of the disinfectant solution were taken every 7 days and assessed quantitatively for bacterial contamination. RESULTS: All automatic procedures prevented recolonisation of the disinfectant solution when a temperature of 60-70°C was ensured for at least 5 min, with or without the addition of chemical cleaning agents. Manual procedures prevented recontamination of the disinfectant solution when rinsing with hot water or a thorough cleaning step was performed before treating all surfaces with an alcohol-based disinfectant or an oxygen-releaser. Other cleaning and disinfection procedures, including the use of an alcohol-based disinfectant, did not prevent recolonisation. CONCLUSIONS: These results indicate that not all processes are effective for processing reusable dispensers for surface-disinfectant tissues, and that a high temperature during the cleaning step or use of a biofilm-active cleaning agent are essential.