2025/03/18 by Marta Vittani, Ashley Bomin Lee, Xiaowen Wang +19 · 1 voice · 2 citations
Biochemistry, Genetics and Molecular Biology · #Biochemical and Structural Characterization
paper · doi:10.1101/2025.03.18.643859
Abstract Sustained labeling of fluids is crucial for their investigation in animal models. Here, we introduce a mouse line (Alb-mSc-ST), where blood and interstitial fluid are labeled with the red fluorescent protein mScarlet and SpyTag. The SpyTag-SpyCatcher technology is exploited to monitor circulating fluid properties by biosensors or detect blood-brain barrier disruption. This approach represents a valuable tool for studying vascular structure, permeability and microenvironment in body organs in vivo .